SCC100

MCF-7/S0.5 Human Breast Cancer Cell Line

Human

• Synonym(e): MCF7 S0.5 ; ; Human breast cancer cell line
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: human

Eigenschaften

• Produktname: MCF-7/S0.5 Human Breast Cancer Cell Line, MCF-7/S0.5 is a tamoxifen-sensitive cell line established from the MCF-7 human breast cancer cell line by adaption to growth in low serum conditions.
• biological source: human
• technique(s): cell culture | mammalian: suitable
• shipped in: ambient
• storage temp.: -140 to -196°C

Beschreibung

General description

Estrogen receptor-positive breast cancer is the most common type of breast cancer. About 80% of all breast cancers express the estrogen receptor (ER+) and depend on estrogen to support the growth and spread of the cancer cells. Hormonal therapy, such as tamoxifen, may help slow or stop the growth of ER+ breast cancers by blocking the actions of estrogen. Primary or acquired tamoxifen resistance poses a major clinical challenge in the treatment of ER+ breast cancer. The MCF-7/S0.5 is the parental line from which many tamoxifen-resistant cell lines have been derived and is utilized as a parental control to its derivative cell lines in parallel experiments.

The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. The MCF-7/S0.5 human breast cancer cells have been adapted to long-term growth in low serum concentration (0.5% fetal bovine serum) by a stepwise reduction of the medium serum content from 5% to 0.5% FBS. MCF-7/S0.5 cells express both estrogen and progesterone receptors and are sensitive to tamoxifen.

Application

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Biochem/physiol Actions

Cancer Cells

Preparation Note

MCF-7/S0.5 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)