00289

Anti-Kaninchen IgG−Abberior^® STAR 512 in Ziege hergestellte Antikörper

for STED application

• Synonym(e): Abberior^® STAR 512-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
• NACRES: NA.32
• UNSPSC Code: 12352200

Eigenschaften

• biological source: goat
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• concentration: ~1 mg/mL
• fluorescence: λ_ex 511 nm; λ_em 530 nm in PBS, pH 7.4
• storage temp.: −20°C

Beschreibung

General description

Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto^™ 514 or Alexa Fluor^™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 511 (PBS pH 7.4), 512 (H₂O), 517 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 85 000 (PBS pH 7.4), 92 000 (H₂O), 95 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,24 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 530 (PBS pH 7.4), 530 (H₂O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

abberior is a registered trademark of Abberior GmbH

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available