408-05A

Human Fibroblast-Like Synoviocytes: HFLS, adult

• Synonym(e): HFLS cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• eCl@ss: 32040201
• Biological source: human synovial tissues (normal)
• Growth mode: Adherent
• Morphology: Fibroblast-like
• Relevant disease(s): arthritis

Eigenschaften

• biological source: human synovial tissues (normal)
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast-like
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): arthritis
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

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Normal HFLS provide an excellent cellular model for studying the normal and pathological physiology of synoviocytes and development of joint diseases.

Normal HFLS have been used in numerous research studies to:

• Study changes in gene expression in synoviocytes stimulated with TNFα (Suggiyama, 2002)
• Demonstrate that TNF-like weak inducer of apoptosis (TWEAK) contributes to joint inflammation by inducing chemokines such as MIP-1β (CCL-4), lymphotactin (XCL-1), IFN-γ-inducible protein 10 (IP-10) (CXCL-10), MCP-1 (CCL-2), and RANTES (CCL-5), as well as the matrix metalloprotease-9, suggesting TWEAK as a new therapeutic target (Perper, 2006)
• Demonstrate the role of miR-124a in arthritis pathogenesis (Nakamachi, 2009)
• Show that C/EBPβ regulates expression of metalloproteinases and ADAMTS family members in synoviocytes stimulated with IL-1β (Tsushima, 2012); and that HMW-HA suppressed ADAMTS4 mRNA and protein expression via CD44, p38 MAPK and JNK pathways (Kataoka, 2013)
• Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as NF-κB inhibitors (Wen, 2006), AGIX-4207 (Kunsch, 2005), HA–methotrexate conjugates (Homma, 2009) and bucillamine (Oki, 2009)
• Show that reactive arthritis triggered by chlamydial infection is mediated by TLR2 induced IL-6 production in synoviocytes (Konomi, 2009); and investigate the mechanisms of arthritis-like syndrome in patients infected with chikungunya (CHIK) virus (Xu, 2013)
• Suggest that only leukocyte-poor, RBC-free platelet-rich plasma should be used in clinical orthopaedics because leukocytes and RBCs cause synoviocyte death and proinflammatory mediator production (Brown, 2014)
• Identify that cartilage link protein and MAGP2 can be used as specific markers to distinguish chondrocytes and synovial cells (Rapko, 2007, 2010)
• Create, along with human dermal fibroblasts, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail”, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takahashi, 2007)

Additionally, in parallel with HFLS isolated from joints of patients with rheumatoid arthritis, normal HFLS were used to:

• Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
• Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
• Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)

Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).

Application

synoviocyte physiology, joint homeostasis, production of collagen, glycoproteins, lubrican, vimentin and hyaluronic acid, gene expression, signal transduction pathways, enzyme production, evaluation of anti-inflammatory agents, effects of extracellular matrix, cell attachment, migration, integrin study, proinflammatory mediator production

Biochem/physiol Actions

Bone

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 5 doublings

Please refer to the HFLS Culture Protocol.

Other Notes

Basal Medium containing 10% FBS & 10% DMSO

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable