41699

Anti-Kaninchen IgG−Abberior^® STAR RED in Ziege hergestellte Antikörper

for STED application

• Synonym(e): Abberior^® STAR RED-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
• NACRES: NA.32
• UNSPSC Code: 12352200

Eigenschaften

• biological source: goat
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• concentration: ~1 mg/mL
• fluorescence: λ_ex 638 nm; λ_em 655 nm in PBS, pH 7.4
• storage temp.: −20°C

Beschreibung

General description

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto^™ 647N, Alexa Fluor^™ 647, or Cy^®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 638 (PBS pH 7.4), 638 (H₂O), 634 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 120 000 (PBS pH 7.4), 125 000 (H₂O), 115 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,16 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 655 (PBS pH 7.4), 655 (H₂O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Features and Benefits

• Unmatched, background free STED imaging cont
• Verified in Abberior Instruments and Leica STED microscopes

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of Cytiva

abberior is a registered trademark of Abberior GmbH

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable