B4429

BIS-TRIS

BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

• Synonym(e): (2,2-Bis-hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis-(2-hydroxyethyl)-amino-2-(hydroxymethyl)-1,3-propandiol, Bis-(2-hydroxyethyl)-amino-tris-(hydroxymethyl)-methan
• Empirische Formel (Hill-System): C₈H₁₉NO₅
• CAS-Nummer: 6976-37-0
• Molekulargewicht: 209.24
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24891775
• EC Number: 230-237-7
• Beilstein/REAXYS Number: 2205275
• MDL number: MFCD00002853

Eigenschaften

• grade: BioPerformance Certified
• Quality Segment: 400
• assay: ≥98.0%
• form: crystalline powder
• technique(s): cell culture | insect: suitable, cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• useful pH range: 5.8-7.2
• pKa (25 °C): 6.5
• mp: 104 °C
• solubility: H₂O: 500 mg/mL, clear, colorless to very faintly yellow
• anion traces: ≤5 ppm
• suitability: suitable for supravital staining of living blood cells
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N

Beschreibung

General description

Bis-Tris is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The useful pH range of Bis-Tris is 5.8 - 7.2.

Application

• A new test for the laboratory diagnosis of spherocytosis: In dieser Studie wird ein neuartiger diagnostischer Assay mit BIS-TRIS-Puffer vorgestellt, der den Nachweis und die Charakterisierung einer Sphärozytose in klinischen Umgebungen verbessern soll. Durch die Stabilität des Puffers und den optimalen pH-Wert ist er für den routinemäßigen Einsatz in klinischen Labors geeignet und trägt zu genauen und reproduzierbaren Diagnoseergebnissen bei (Vettore et al., 1984).

Features and Benefits

Features:

1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
3. M-Clarity^™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity^™ Program
4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
   1. Site quality assessment
   2. ISO 9001 certificate
   3. Detailed product specifications
   4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)

Benefits:

1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Other Notes

Die BIS-TRIS-Spezifikationstabelle ermöglicht einen einfachen Vergleich der Spezifikationen verschiedener BIS-TRIS-Produkte.

Legal Information

M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves