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C2639

Cell Freezing Medium-Serum-free 1×

sterile-filtered, suitable for cell culture

Synonym(e):

Cryopreservation Medium, Serum-Free Freezing Medium

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Größe/SKUVerfügbarkeitPreis
50 mL
Warenkorb auf Verfügbarkeit prüfen
€ 256,00

Über diesen Artikel

NACRES:
NA.75
UNSPSC Code:
12352207

€ 256,00

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Unterstützung erhalten

sterility

sterile-filtered

Quality Segment

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

Application

Cell Freezing Medium-Serum-free 1× has been used as a cryopreservation medium for somatic cell sampling and recovery (SCSR) exfoliated cells cultured in growth media and dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cells grown in serum-free growth medium.
Sigma′s Cell Freezing Medium, Serum-free is a cryopreservation medium which does not contain DMSO or serum.

Other Notes

Ready-to-use formula that does not contain dimethyl sulfoxide or serum.

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Dieser Artikel
C6295C6164C6039
Cell Freezing Medium-Serum-free 1× sterile-filtered, suitable for cell culture

Sigma-Aldrich

C2639

Cell Freezing Medium-Serum-free 1×

Zellkultur-Einfriermedium DMSO, serumfrei, 1x sterile-filtered, suitable for cell culture

Sigma-Aldrich

C6295

Zellkultur-Einfriermedium DMSO, serumfrei, 1x

Zellgefriermedium-DMSO 1× sterile-filtered, suitable for cell culture

Sigma-Aldrich

C6164

Zellgefriermedium-DMSO 1×

Sigma-Aldrich

Sigma-Aldrich

C6039

Cell Freezing Medium-Glycerol 1×

description

sterile-filtered, suitable for cell culture

description

sterile-filtered, suitable for cell culture

description

sterile-filtered, suitable for cell culture

description

sterile-filtered, suitable for cell culture

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cryopreservation: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cryopreservation: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

Lagerklasse

10 - Combustible liquids

Was ist los?

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

  1. Hi there, can i use this C2639 Cell Freezing Medium-Serum-free 1× for freezing HEK293 and MCF7 cells? In addition, can i use it to freeze hESC and hiPSC?

    1 answer

    1. This Cell Freezing Medium-Serum-free 1× does not contain dimethyl sulfoxide or serum and may not be suitable for all cell types. It is recommended to follow manufacturers' guidelines or literature for cell freezing. For example:

      - Human kidney (embryonic) 293 cells, Cat. #85120602-1VL: 5% dimethyl sulfoxide and 95% fetal calf serum.
      - MCF7 cells: 95% complete growth medium and 5% dimethyl sulfoxide.
      - Simplicon™ Human iPS cells: pluripotent media with 10% dimethyl sulfoxide.
      - Human embryonic stem cells: base medium with fetalbovine serum and 10% dimethyl sulfoxide.

      For cryopreservation of human embryonic stem cells or induced pluripotent stem cells in serum-free conditions, PluriSTEM™ Freeze Medium (SCM134) is recommended.

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