VP229 Human Breast, ECACC 05092804

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5092804, human breast, Epithelial

• UNSPSC Code: 41106514
• eCl@ss: 32160903
• Biological source: human breast
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Mean no. 74, modal no. 66, range 55-134
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• Produktname: VP229, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5092804, human breast, Epithelial
• biological source: human breast
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 05092804-DNA-5UG, pkg of vial of cells 05092804-1VL
• growth mode: Adherent
• karyotype: Mean no. 74, modal no. 66, range 55-134
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Derived from a breast cancer biopsy removed from a 47 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade III, tumour cells did not stain positive for oestrogen receptors. The VP229 cell line was derived from the same individual as the cell line VP267. VP229 was derived from the primary tumour and VP267 was derived 14 months later from a local recurrence of the tumour. The individual was recorded to live for 2 years after surgery performed to remove the primary tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

Human Breast Cancer

Preparation Note

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.