D3818

Dextran Ladder

Glycan standard for HPLC

• UNSPSC Code: 12352201

Eigenschaften

• biological source: bacterial (Leuconostoc mesenteroides)
• form: solid
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Application

The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. This standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide, dependent upon the chromatographic conditions employed. The elution position of each peak in this ladder is expressed as a glucose unit (gu) and is used to assign gu values to peaks in the released glycan pool.Dextran ladder upon derivitization can be used as a new tool to assess cell layer tightness.

Preparation Note

The dextran ladder is prepared by partial acid hydrolysis of dextran from Leuconostoc mesenteroides with an average molecular weight of 100-200 kDa. The purity and structural integrity of the ladder is assessed by fluorescently labeling an aliquot and subsequent analysis by normal phase HPLC.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable