D5282

Lambda DNA EcoR I Digest

for DNA electrophoresis

• NACRES: NA.25
• UNSPSC Code: 12352200

Eigenschaften

• form: liquid
• usage: 100 uses
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Beschreibung

General description

Sigma′s Lambda DNA EcoR I Digest contains 6 fragments from 3,530 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I to completion, then purified by phenol extraction.

Other Notes

Contains 6 fragments, 3,530–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Sigma′s Lambda DNA EcoR I Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable