E1131

Exonuclease III from Escherichia coli BE25 /psGR3

buffered aqueous glycerol solution

• CAS-Nummer: 9037-44-9
• UNSPSC Code: 12352204
• EC Number: 232-912-1
• MDL number: MFCD00163580
• EG-Nummer: 3.1.11.2 (BRENDA, IUBMB)

Eigenschaften

• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• mol wt: 28 kDa
• UniProt accession no.: P09030
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... xthA(946254)

Beschreibung

General description

A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.

Application

Suitable for:

• Production of strand specific probes
• Preparation of single stranded templates for Sanger dideoxy sequencing
• Site directed mutagenesis

Other Notes

Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.

One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)