F5884

α-L-Fucosidase from bovine kidney

ammonium sulfate suspension, ≥2.0 units/mg protein (biuret)

• Synonym(e): α-L-Fucoside fucohydrolase
• UNSPSC Code: 12352204
• NACRES: NA.32
• EG-Nummer: 3.2.1.51 (BRENDA, IUBMB)
• MDL number: MFCD00130491

Eigenschaften

• biological source: bovine kidney
• form: ammonium sulfate suspension
• specific activity: ≥2.0 units/mg protein (biuret)
• mol wt: 210-220 kDa (Subunits: 55-60 kDa)
• foreign activity: α-mannosidase and β-galactosidase ≤0.1%, β-N-acetylglucosaminidase ≤0.2%
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

α-L-fucosidases is a liposomal enzyme, which belongs to glycosyl hydrolases family.

Application

α-L-fucosidase from bovine kidney has been used:

• to study their ability to alter primary cell transduction
• to examine the role of fucose residues of cell surface glycans in adhesion
• to investigate its role during fertilization

Biochem/physiol Actions

α-L-fucosidases degrades fucose-containing fucoglycoconjugates and breaks fucosidic bonds in oligosaccharides and glycoconjugates. It acts as a tumor marker for hepatic and colorectal cancer. α-L-fucosidase exhibits transglycosylation property.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 10 mM NaH₂PO₄ 10 mM citrate, pH 6.0

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-L-fucoside to p-nitrophenol and L-fucose per min at pH 5.5 at 25°C.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable