1.15444

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

• Synonym(s): Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
• Empirical Formula (Hill Notation): C₄₇H₄₈N₃NaO₇S₂
• CAS Number: 6104-58-1
• Molecular Weight: 854.02
• MDL number: MFCD00078482
• UNSPSC Code: 41105305
• EC Index Number: 228-058-4
• NACRES: NC.01

Properties

• Quality Level: 100
• potency: >5000 mg/kg LD₅₀, oral (Rat)
• technique(s): protein staining: suitable
• pH: 6.4 (20 °C, 10 g/L in H₂O)
• solubility: 40 g/L
• bulk density: 520 kg/m³
• storage temp.: no temp limit
• SMILES string: [Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2cc(c(cc2)[C+](c5c(cc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])C)c3ccc(cc3)Nc4ccc(cc4)OCC)C
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
• InChI key: CMGWKYXJIYYESN-UHFFFAOYSA-M

Description

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye.[1] CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[2] CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.[1]

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[3] It has also been used in Bradford protein assay.[4]

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

Legal Information

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable