To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.
12-342
Rabbit Anti-Sheep IgG Antibody, HRP conjugate
Upstate®, from rabbit
Sign In to View Organizational & Contract Pricing
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Skip To
biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
sheep
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
1 of 4
This Item | 12-349 | AP180P | AP124C |
|---|---|---|---|
| species reactivity sheep | species reactivity mouse | species reactivity goat | species reactivity mouse |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate CY3 conjugate |
| biological source rabbit | biological source goat | biological source donkey | biological source goat |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form F(ab′)2 fragment of affinity isolated antibody |
| technique(s) ELISA: suitable, western blot: suitable, immunohistochemistry: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) immunofluorescence: suitable |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Application
Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Physical form
0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate
Preparation Note
Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.
Analysis Note
Routinely evaluated by western blot
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Donel A Sequea et al.
Mechanisms of ageing and development, 134(1-2), 60-63 (2012-12-19)
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence
Susannah L McKay et al.
PloS one, 6(1), e16077-e16077 (2011-02-02)
There is mounting evidence to suggest that the synthesis of pre-mRNA transcripts and their subsequent splicing are coordinated events. Previous studies have implicated the mammalian spliceosomal U2 snRNP as having a novel role in stimulating transcriptional elongation in vitro through
Arvin Bansal et al.
Journal of proteomics, 73(10), 2041-2049 (2010-07-06)
Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric
Naveen Sharma et al.
PloS one, 8(6), e65118-e65118 (2013-06-12)
Calorie restriction (CR) (consuming ~60% of ad libitum, AL, intake) improves whole body insulin sensitivity and enhances insulin-stimulated glucose uptake by isolated skeletal muscles. However, little is known about CR-effects on in vivo glucose uptake and insulin signaling in muscle.
Naveen Sharma et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(12), R1261-R1267 (2012-11-02)
Akt is a serine/threonine kinase that plays a key role in numerous cellular functions including metabolism, growth, protein synthesis, apoptosis, and cell proliferation. The most consistent and robust effect of moderate calorie restriction (CR; ~60% of ad libitum, AL, food
-
Could you please provide the reconstitution method for this product and the recommended concentration for various uses?
1 answer-
Helpful?
-
-
What is the method of reconstitution for this product and what is the concentration recommended for various uses?
1 answer-
This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service