219364-M

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• EC Number: 3.4.22.1 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• MDL number: MFCD00406994

Properties

• Product Name: Cathepsin B, Human Liver, Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.
• biological source: human liver
• description: Merck USA index - 14, 1905
• assay: >95% (SDS-PAGE and HPLC)
• form: liquid
• specific activity: ≥50 units/mg protein
• purified by: affinity chromatography
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): activity assay: suitable
• pH range: 3.5-6.0
• suitability: suitable for molecular biology
• application(s): life science and biopharma
• shipped in: wet ice
• storage temp.: −70°C
• Quality Level: 100
• Gene Information: human ... CTSB(1508)

Description

Application

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.[1][2]

Biochem/physiol Actions

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. [3]Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Disclaimer

Toxicity: Standard Handling (A)

General description

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidasecitation and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[3]

Optimal pH: 3.5-6.0.

Other Notes

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286. Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281. Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117. Musil, D., et al. 1991. EMBO J.10, 2321. Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany