The beads are not characterized in terms of beads/ml but only in terms of a percentage slurry (10%, weight [g] by volume [mL]).
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€ 320,00
€ 983,00
€ 320,00
About This Item
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Product Name
PureProteome Protein A Magnetic Bead System, The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability
packaging
pkg of 2 × 1 mL
manufacturer/tradename
PureProteome
technique(s)
depletion: suitable (serum)
immunoprecipitation (IP): suitable
protein purification: suitable
particle size
10 μm
capacity
1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)
shipped in
wet ice
Quality Level
1 of 4
This Item | LSKMAGG | LSKMAGH | LSKMAGAG |
|---|---|---|---|
| packaging pkg of 2 × 1 mL | packaging pkg of 2 × 1 mL | packaging pkg of 10 mL | packaging pkg of 2 × 1 mL |
| manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome |
| technique(s) depletion: suitable (serum) | technique(s) depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) protein purification: suitable (histidine tagged recombinant protein) | technique(s) immunoprecipitation (IP): suitable (crosslinking), protein purification: suitable (antibody) |
| particle size 10 μm | particle size 10 μm | particle size 10 μm | particle size 10 μm |
| capacity 1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG) | capacity 2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG) | capacity 1-5.5 mg/mL, bead suspension binding capacity (protein) | capacity 2-3 mg/mL, suspension binding capacity (rabbit IgG) |
| shipped in wet ice | shipped in wet ice | shipped in ambient | shipped in wet ice |
Application
- in serum depletion, in immunoprecipitation, for protein purification, and in cell lysates to analyze the bound proteins by western blot[1]
- in magnetic bead-binding assays[1]
- for immunoprecipitating cell lysate and antibodies for ATPase activity[1]
Disclaimer
General description
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
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