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Merck

DIUTP-RO

Roche

Digoxigenin-11-dUTP, alkali-labile

=85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])

Synonym(s):

Digoxigenin-11-dUTP, alkali-labile, digoxigenin

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UNSPSC Code:
41105500

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Quality Level

100

Assay

85% (HPLC)

form

solution

packaging

pkg of 125 μL (11573179910 [1 mM]]])
pkg of 25 μL (11573152910 [1 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

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This Item
DIUTPS-RO11363905910DIGUTP-RO
Digoxigenin-11-dUTP, alkali-labile =85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])

Roche

DIUTP-RO

Digoxigenin-11-dUTP, alkali-labile

Digoxigenin-11-dUTP, alkali-stable

Roche

DIUTPS-RO

Digoxigenin-11-dUTP, alkali-stable

Digoxigenin-11-ddUTP solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

Roche

11363905910

Digoxigenin-11-ddUTP

Digoxigenin-11-UTP

Roche

DIGUTP-RO

Digoxigenin-11-UTP

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

assay

85% (HPLC)

assay

≥85% (HPLC)

assay

85% (HPLC)

assay

≥85% (HPLC)

form

solution

form

solution

form

solution

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM])

packaging

pkg of 125 μL (11558706910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]), pkg of 25 μL (11093088910 [1 mM])

packaging

pkg of 25 μL (25 nmol; 1mM)

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

General description

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.

Application

Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Other Notes

For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
10739639
10739637
10739635
10739659
10739653

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