The preferred fixative for various staining procedures is often freshly prepared paraformaldehyde solution. Paraformaldehyde solution is not known to quench fluorescein sodium salt or other fluorescent molecules. However, no specific analysis been performed as part of the quality control testing performed on product F6377 to ensure fluorescence is not quenched.
F6377
Fluorescein sodium salt
Powder
Fluorescein sodium salt
Synonym(s):
Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
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About This Item
Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
208-253-0
MDL number:
Colour Index Number:
45350
Beilstein/REAXYS Number:
3833041
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Product Name
Fluorescein sodium salt, used as fluorescent tracer
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
SMILES string
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI key
RGPLGPBQJOQFJS-UHFFFAOYSA-L
form
powder
technique(s)
titration: suitable
pH
8.3 (20 °C, 10 g/L)
mp
>300 °C
solubility
H2O: 1 mg/mL
density
0.78 g/cm3 at 29.6 °C (85.3 °F at 699.7 hPa)
fluorescence
λex 460 nm; λem 515 nm(lit.)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | 46970 | 1.03992 | 1.03887 |
|---|---|---|---|
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) - |
| fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - | fluorescence - |
| color orange to brown | color - | color - | color red-brown |
| solubility H2O: 1 mg/mL | solubility - | solubility 500 g/L | solubility 500 g/L |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) pharma/biopharma processes | application(s) - |
| form powder | form powder | form powder | form powder |
Application
Fluorescein sodium salt has been used as a solute to study microvessel permeability.[1][2] It has been used as a test molecule to study blood barrier penetration.[3]
Water soluble fluorescent dye used in permeability and vascular perfusion studies due to its small molecular radius.[4] Sodium fluorescein is used as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models.[5][6][7] The dye was used as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes.[8] Sodium fluorescein was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP).[9]
General description
Fluorescein is a xanthene dye and is a common labeling agent in biology.[10]
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
423.7 °F - Pensky-Martens closed cup
flash_point_c
217.6 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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The acute and chronic toxicity of two xanthene dyes, fluorescein sodium salt and phloxine B, to Daphnia pulex.
Walthall WK and Stark JD.
Environmental Pollution (Barking, Essex : 1987), 104(2), 207-215 (1999)
Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design (2010)
Vascular endothelial growth factor enhances cancer cell adhesion to microvascular endothelium in vivo.
Shen S
Experimental Physiology, 95, 369-369 (2010)
Dimitris N Xanthos et al.
Molecular pain, 8, 44-44 (2012-06-21)
Changes in the blood-central nervous system barriers occur under pathological conditions including inflammation and contribute to central manifestations of various diseases. After short-lasting peripheral and neurogenic inflammation, the evidence is mixed whether there are consistent blood-spinal cord changes. In the
Liyun Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(33), 11911-11916 (2005-08-10)
Solute transport through the bone lacunar-canalicular system is believed to be essential for osteocyte survival and function but has proved difficult to measure. We report an approach that permits direct measurement of real-time solute movement in intact bones. By using
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Hello, I would like to ask if this product's fluorescence can be quenched by paraformaldehyde (PFA)? Thank you.
1 answer-
Helpful?
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What is the purity of Fluorescein sodium salt (CAS number 518-47-8, article number F6377-100G)?
1 answer-
This purity of this product is not determined.
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Can this product be autoclaved once dissolved? How long can a concentrated dissolved stock solution be stored for?
1 answer-
The stability of Fluorescein under autoclave conditions has not been determined. Solutions have typically been filter sterilized. Long term solution stability has also not been evaluated. However, solutions of Fluorescein Diacetate are stable at -20°C in ethanol when protected from light. Similar stability would be expected with the sodium salt form.
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Hello, I am interested in Fluorescein or Fluoresein sodium salt to perform angiography in the living mouse and would like to know if your product has a pharmacological grade. Which product is used for angiography?
1 answer-
Unfortunately, a pharmacological grade Fluorescein or Fluorescein sodium salt is not available at this time.
Helpful?
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Is F6377 soluble in ethanol?
1 answer-
The substance is soluble in water (500 g/l) at 20°C and slightly soluble in ethanol, but insoluble in benzene, chloroform, and ether.
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