The kit is intended for the isolation of intact mitochondria, so most of the isolated mitochondria will contain intact inner and outer membranes. While trace amounts of mitochondria-associated membranes (MAMs) may co-purify during the process, the protocol is not optimized to enrich or preserve MAMs. Please see the Product Information Sheet at the link below for further details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/204/819/mitoiso1pis-ms.pdf
MITOISO1
Mitochondria Isolation Kit
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Synonym(s):
Mitochondria Purification Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | € 900,00 |
About This Item
UNSPSC Code:
12352200
EC Number:
232-650-8
NACRES:
NA.32
€ 900,00
Check Cart for Availability
Quality Segment
usage
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL)
shelf life
2 yr
technique(s)
fractionation: suitable
input
mammalian sample(s)
shipped in
dry ice
storage temp.
−20°C
Application
Mitochondria Isolation Kit has been used:
- for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
- for the isolation of mitochondria from glomeruli and heart
- for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential
The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Features and Benefits
- Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
- Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
- Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
- Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues | description sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells | description 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates) | description 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions) |
| technique(s) fractionation: suitable | technique(s) ELISA: suitable, western blot: suitable | technique(s) protein staining: suitable, flow cytometry: suitable | technique(s) protein staining: suitable, flow cytometry: suitable |
| usage sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL) | usage sufficient for 50 applications (2-5 x 107 cells) | usage kit sufficient for 50 reactions (in a 2 mL cuvette), kit sufficient for 1000 reactions (using 96 multiwell plates) | usage kit sufficient for 40 tests (of 5 mL cell suspensions), kit sufficient for 200 tests (of 1 mL cell suspensions) |
| input mammalian sample(s) | input mammalian sample(s) | input - | input - |
| shelf life 2 yr | shelf life - | shelf life - | shelf life - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
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Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
10 - Combustible liquids
WGK
WGK 3
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Is it possible to use the MITOISO1 kit to also isolate MAMs?
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Where is the step to get cytosol?
1 answer-
The kit is intended for the isolation of intact mitochondria. It was not developed for cytoplasma collection. Nevertheless, reviewing the protocol, it can be assumed that the cytoplasmic fraction should be in the supernatant after the second centrifugation (point 8 of the Preparation of mitochondria from soft tissues protocol). Please keep in mind that this is not tested for suitability for cytoplasmic fraction.
Please see product MIT1000 Mitochondria/Cytosol Fractionation Kit for efficient isolation of intact mitochondria from cultured cells. This kit can be used for tissue samples as long as the tissue is first homogenized into a single cell suspension. It may be helpful to use the same protocol that is recommended for cells following homogenization of the tissue into a single cell suspension in the supplied Isotonic Mitochondrial Buffer containing protease inhibitors, then follow the kits protocol for cells: https://www.sigmaaldrich.com/product/mm/mit1000.
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How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?
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The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin. We recommend a minimum of 50 mg.
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When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?
1 answer-
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?
1 answer-
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose. This kit was originally designed for isolation from tissues.
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Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?
1 answer-
The extraction buffers supplied with this kit are only available with the kit.
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Is the Product MITOISO1, Mitochondria Isolation Kit, JC-1 stain available separately?
1 answer-
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.
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When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?
1 answer-
If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
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