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MIT1000

Mitochondria/Cytosol Fractionation Kit

This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

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1 kit
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$686.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.75
eCl@ss:
32161000

$686.00

Check Cart for Availability
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Quality Segment

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

application(s)

sample preparation

shipped in

dry ice

General description

Mitochondria, sometimes described as the “power plants” of the cell, are sites where most of the energy production in eukaryotic cells takes place. The synthesis of most of the adenosine triphosphate (ATP) occurs in these double membraned organelles that are found in living cells. ATP production by the mitochondria is done by the process of respiration, which uses oxygen to generate energy. This is a very efficient process for using food energy to make ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria also regulate crucial apoptosis signaling pathways. The number of mitochondria in a cell varies widely by organism and tissue type. Many cells have only a few mitochondria, whereas others can contain several thousand.

Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.

The kit contains sufficient materials for the analysis of 50 samples.

Application

Research Category
Apoptosis & Cancer

Preparation Note

All components should be store at -20°C for up to one year from date of receipt.

Other Notes

Isotonic Mitochondrial Buffer (CS204255): 50 mL

Mitochondrial Lysis Buffer (CS204257): 5 mL

Protease inhibitor cocktail (CS204253): 1 mL

Anti-Bcl2 (05-729-25UG): 25 μg

Anti-GAPDH (CS204254): 25 μg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
MITOISO1MITOISO2CS0760
Mitochondria/Cytosol Fractionation Kit This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

Sigma-Aldrich

MIT1000

Mitochondria/Cytosol Fractionation Kit

Mitochondria Isolation Kit sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Sigma-Aldrich

MITOISO1

Mitochondria Isolation Kit

Mitochondria Isolation Kit sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sigma-Aldrich

MITOISO2

Mitochondria Isolation Kit

Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Sigma-Aldrich

CS0760

Isolated Mitochondria Staining Kit

description

This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

description

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

description

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

description

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

technique(s)

cell culture | mammalian: suitable

technique(s)

fractionation: suitable

technique(s)

western blot: suitable, fractionation: suitable, ELISA: suitable

technique(s)

protein staining: suitable, flow cytometry: suitable

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

application(s)

sample preparation

application(s)

-

application(s)

-

application(s)

cell analysis
detection

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

species reactivity (predicted by homology)

all

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H315,H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

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