The product is provided in powder form. To ensure proper functioning of the solution, it is necessary to adjust the solution to achieve a pH of 2.5. Typically, a 3% acetic acid solution should approximate pH 2.5.
A5268
Alcian Blue 8GX
powder
Synonym(s):
Alcian Blue, Ingrain Blue 1
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10 G
€ 293,00
25 G
€ 584,00
100 G
€ 1.910,00
About This Item
Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
251-705-7
Colour Index Number:
74240
MDL number:
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Product Name
Alcian Blue 8GX, powder
InChI key
CKLBXIYTBHXJEH-UHFFFAOYSA-J
InChI
1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
SMILES string
[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
form
powder
composition
Dye content, 45-65%
technique(s)
microbe id | staining: suitable
color
blue
solubility
H2O: 1 mg/mL
εmax
425 at 615-630 nm in water
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Related Categories
1 of 4
This Item | 05500 | A3157 | 335630 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| color blue | color - | color dark blue | color - |
| form powder | form powder | form powder | form powder or crystals |
| solubility H2O: 1 mg/mL | solubility methanol: water (1:1): 0.01 g/10 mL, blue to very deep blue | solubility water: 1 mg/mL, blue | solubility water: 1 mg/mL |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
Application
- Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.[1][2]
- It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.[3]
- It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.[4]
Biochem/physiol Actions
The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.
Features and Benefits
Desired staining patterns can be obtained by manipulating pH and salt concentrations.[5]
General description
Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.[5]
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Find documentation for the products that you have recently purchased in the Document Library.
In vivo visualization of Bonghan ducts inside blood vessels of mice by using an Alcian blue staining method
Yoo, et al.
Indian Journal of Experimental Biology, 46 (2008)
Replacement of the medial tibial plateau by a metallic implant in a goat model.
Custers RJ, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 28, 429-435 (2010)
Selective COX-2 inhibition prevents proinflammatory cytokine-induced cartilage damage.
Mastbergen SC, et al.
Rheumatology (Oxford, England), 41, 801-808 (2002)
Exposure of human cartilage tissue to low concentrations of blood for a short period of time leads to prolonged cartilage damage: an in vitro study.
Jansen NW, et al.
Arthritis and Rheumatism, 56, 199-207 (2007)
Alcian Blue and Pyronine Y Histochemical Stains Permit Assessment of Multiple Parameters in Pulmonary Disease Models
Meyerholz, et al.
Veterinary Pathology, 46 (2009)
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Is it normal for the pH of A5268 to be 1.5 instead of the usual 2.5?
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