B4429

BIS-TRIS

BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

• Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
• Empirical Formula (Hill Notation): C₈H₁₉NO₅
• CAS Number: 6976-37-0
• Molecular Weight: 209.24
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24891775
• EC Number: 230-237-7
• Beilstein/REAXYS Number: 2205275
• MDL number: MFCD00002853

Properties

• Product Name: BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• grade: BioPerformance Certified
• assay: ≥98.0%
• form: crystalline powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• useful pH range: 5.8-7.2
• pKa (25 °C): 6.5
• mp: 104 °C
• solubility: H₂O: 500 mg/mL, clear, colorless to very faintly yellow
• anion traces: ≤5 ppm
• suitability: suitable for supravital staining of living blood cells
• application(s): diagnostic assay manufacturing
• Quality Level: 400

Description

Application

• A new test for the laboratory diagnosis of spherocytosis: This study introduces a novel diagnostic assay utilizing BIS-TRIS buffer, aimed at improving the detection and characterization of spherocytosis in clinical settings. The buffer′s stability and optimal pH make it suitable for routine use in clinical laboratories, contributing to accurate and reproducible diagnostic results (Vettore et al., 1984).

Other Notes

Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

General description

Bis-Tris is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The useful pH range of Bis-Tris is 5.8 - 7.2.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves