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CHOGS

CHOZN® ZFN-Modified CHO Cell Line

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UNSPSC Code:
12352200
NACRES:
NA.75

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description

CHOZN® GS-/- ZFN-modified CHO cell line
glutamine synthetase knockout cell line

solubility

DMSO: 5 mg/mL, clear

shipped in

liquid nitrogen

storage temp.

−196°C

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This Item
CHODHFRCHOK1SCC494
CHOZN® ZFN-Modified CHO Cell Line

SAFC

CHOGS

CHOZN® ZFN-Modified CHO Cell Line

CHOZN® ZFN-Modified CHO Cell Line

SAFC

CHODHFR

CHOZN® ZFN-Modified CHO Cell Line

CHOZN® CHO K1 Host Cell Line Suspension-adapted in CD media

SAFC

CHOK1

CHOZN® CHO K1 Host Cell Line

Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

Sigma-Aldrich

SCC494

Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

shipped in

liquid nitrogen

shipped in

dry ice

shipped in

dry ice

shipped in

liquid nitrogen

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

description

CHOZN® GS-/- ZFN-modified CHO cell line

description

CHOZN® DHFR-/- ZFN-modified CHO cell line, dihydrofolate reductase knockout cell line

description

CHOK1 cell line derived from ECACC CHO K1

description

-

solubility

DMSO: 5 mg/mL, clear

solubility

-

solubility

-

solubility

-

General description

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media
ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus.[1] Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth.[2] The GS enzyme is responsible for the conversion of glutamate into glutamine.[3] Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Biochem/physiol Actions

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

Packaging

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Physical form

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Legal Information

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
2113545207
2113545207
2113-53667
051M0924

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Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer.
DeBerardinis R J and Cheng T
Oncogene, 29(3), 313-313 (2010)
The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type.
Yuneva M O, et al.
Cell Metabolism, 15(2), 157-170 (2012)
Cytotoxic effects during knock out of multiple porcine endogenous retrovirus (PERV) sequences in the pig genome by zinc finger nucleases (ZFN).
Semaan M, et al.
PLoS ONE, 10(4), e0122059-e0122059 (2015)

Articles

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

CHO Media and Feeds for Optimized Production of Therapeutic Proteins

Cellvento® and EX-CELL® CHO cell culture media and feeds, optimized for monoclonal antibody & recombinant protein production in fed-batch & perfusion

Optimizing CHO Cells for Higher Productivity

Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

See All

Related Content

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CHO cell genome editing

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Accelerate development and optimization of cell culture media for upstream perfusion processes

This page describes the use of a small-scale perfusion bioreactor with integrated microfluidic flow control and sensor technologies to predict large-scale processes.

Glycosylation Modulation in Fed-batch Production of Therapeutic Proteins

Enhance mAb and recombinant protein performance, efficacy, and stability with precise glycosylation modulation using the Cellvento® ModiFeed platform.

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