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P1415

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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Size/SKUAvailabilityPrice
50 mL
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€ 176,00
5 x 50 mL
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€ 698,00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

€ 176,00


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Quality Segment

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

-10 to -25°C

General description

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Application

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Preparation Note

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

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This Item
H7140D9905D2532
description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, lyophilized powder

description

for Northern and Southern blotting, solution

Quality Level

100, 200

Quality Level

100

Quality Level

100

Quality Level

300

form

liquid (2x concentrate)

form

liquid (2x concentrate)

form

lyophilized powder

form

solution

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected


Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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