RNA Marker sample solutions for electrophoresis should be prepared as follows: Mix 2-4 µl of RNA Marker, add water (Rnase free) to make up to 5 µl, then add 3 µl of RNA Sample Loading Buffer, and 1 µl of 200 mM Potassium Acetate, pH 4.5. Additional information can be found in the Product Information Sheet.
R7020
Transcript RNA Markers 0.2-10 kb
for RNA electrophoresis
Synonym(s):
RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker
Sign In to View Organizational & Contract Pricing
Select a Size
50 μG
€ 381,00
€ 381,00
Check Cart for Availability
About This Item
UNSPSC Code:
41105337
NACRES:
NA.25
Skip To
grade
Molecular Biology
Quality Level
form
liquid
usage
25 uses
solubility
water: soluble
suitability
suitable for electrophoresis (RNA)
storage temp.
−70°C
1 of 4
This Item | R7644 | P9577 | D7058 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| usage 25 uses | usage 25 uses | usage sufficient for 50 uses | usage 100 uses |
| suitability suitable for electrophoresis (RNA) | suitability suitable for electrophoresis (RNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
General description
Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Application
Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.[1]
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.[1]
Other Notes
Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.
Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch.
Wright SA, et al.
Plant Pathology, 62, 982-992 (2013)
Magda Dudek et al.
Nucleic acids research, 48(14), 7786-7800 (2020-06-26)
Marine flavobacteria possess dedicated Polysaccharide Utilization Loci (PULs) enabling efficient degradation of a variety of algal polysaccharides. The expression of these PULs is tightly controlled by the presence of the substrate, yet details on the regulatory mechanisms are still lacking.
Articles
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.
Related Content
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Werkzeuge für die Plasmidpräparation, in vitro Transkription, mRNA-Reinigung und LNP-Formulierung in der mRNA-basierten Impfstoff- und Therapieentwicklung.
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
-
in what proportion should i mix RNA marker and loading dye buffer.
1 answer-
Helpful?
-
-
Can I denaturate it using Glyoxal?
1 answer-
This product has been tested on 1% agarose gel with formaldehyde. It has not been specifically qualified for use with glyoxal, however there is no reason to suspect that it would not be suitable. See the reference in the link below comparing formaldehyde to glyoxal:
https://link.springer.com/protocol/10.1385/0-89603-127-6:1Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service