Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)

E Brindha, M Rajasekapandiyan

ABSTRACT

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIALS

Product Number

Brand

Product Description

PHR1321

Supelco

Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material

RDD040

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

258148

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

304174

Sigma-Aldrich

Hydrogen chloride solution, 1.0 M in acetic acid

320331

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

294837

Sigma-Aldrich

Hydrogen chloride solution, 1.0 M in diethyl ether

339253

Sigma-Aldrich

Hydrochloric acid, 37 wt. % in H₂O, 99.999% trace metals basis

455180

Sigma-Aldrich

Hydrogen chloride solution, 2.0 M in diethyl ether

345547

Sigma-Aldrich

Hydrogen chloride solution, 4.0 M in dioxane

07102

Sigma-Aldrich

Hydrochloric acid, meets analytical specification of Ph. Eur., BP, NF, fuming, 36.5-38%

17934

Supelco

Hydrogen chloride – ethanol solution, ~1.25 M HCl, derivatization grade (GC derivatization), LiChropur^™

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

S3014

Sigma-Aldrich

Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

S1679

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

S6150

Sigma-Aldrich

Sodium chloride, tablet

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

2104

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

M8787

Sigma-Aldrich

Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂solution for PCR

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

H9892

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

M1028

Sigma-Aldrich

Magnesium chloride solution, Molecular Biology, 1.00 M±0.01 M

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

H1758

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, Molecular Biology

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

17933

Supelco

Hydrogen chloride – 2-propanol solution, ~1.25 M HCl (T), derivatization grade (GC derivatization), LiChropur^™

71376

Sigma-Aldrich

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)