Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Chemical & pharmaceutical bulletin (2015-12-04)

Yoon Kyung Kwon, Soo Jung Choi, Cho Rong Kim, Jae Kyeom Kim, Hye Kyung Kim, Jong Hun Choi, Sang-Wook Song, Chang-Ju Kim, Gwi Gun Park, Cheung-Seog Park, Dong-Hoon Shin

PMID26633030

ABSTRACT

The aim of this study was to search for a novel choline acetyltransferase (ChAT) activator from plants traditionally grown in Korea. An ethanol extract from Chaenomeles sinensis Koehne showed the highest ChAT-activating effect in vitro in an assay that used human neuroblastoma cells and [(14)C]acetyl-CoA. The active compound was speculated to be stearic acid methyl ester (SAME). In an in vivo experiment, C. sinensis extract and SAME improved trimethyltin (TMT)-induced deficits in learning and memory in mice as assessed by a Y-maze behavioral test and a passive avoidance test. The C. sinensis extract might attenuate the TMT-induced brain disorder. This study suggests that SAME from C. sinensis might be useful in the treatment of Alzheimer's disease.

MATERIALS

Product Number

Brand

Product Description

E7148

Sigma-Aldrich

Ethyl alcohol, Pure 190 proof, for molecular biology

204439

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

378860

Sigma-Aldrich

Sodium chloride, random crystals, 99.9% trace metals basis

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

S0817

Sigma-Aldrich

Sodium chloride solution, 0.85%

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

S3014

Sigma-Aldrich

Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S1679

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S6150

Sigma-Aldrich

Sodium chloride, tablet

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

59222C

SAFC

Sodium chloride solution, 5 M

71376

Sigma-Aldrich

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H₂O

S6191

Sigma-Aldrich

Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

T25402

Sigma-Aldrich

Sodium tetraphenylborate, ACS reagent, ≥99.5%

431788

Sigma-Aldrich

Ethylenediaminetetraacetic acid, 99.995% trace metals basis

459836

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

C1879

Sigma-Aldrich

Choline chloride, ≥98%

C7017

Sigma-Aldrich

Choline chloride, ≥99%

EDS

Sigma-Aldrich

Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)

E9884

Sigma-Aldrich

Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder

E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture

E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder

244511

Sigma-Aldrich

Toluene, anhydrous, 99.8%