HPLC Analysis of 2-Chloroethanol Content in Hydroxyzine HCl on SeQuant^® ZIC-HILIC with Refractive Index Detection

column

SeQuant ZIC-HILIC 150 X 4.6 mm, 5.0 uM, 200 Angstrom (1.51455.0001)

mobile phase

[A] 40mM ammonium acetate; [B] acetonitrile;; (25:75, A:B)

flow rate

0.5 mL/min

column temp.

40 °C

detector

Shimadzu Proninance, RI (Detection Range 1, Cell temp. 40 °C

injection

25 μL

sample

100 ppm