- Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.
Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.
Bio-medical materials and engineering (2014-09-18)
Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao
PMID25226889
ABSTRACT
To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester, ≥98%, powder
Sigma-Aldrich
Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer
Sigma-Aldrich
Dichloromethane, biotech. grade, 99.9%, contains 40-150 ppm amylene as stabilizer