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Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Talanta (2015-11-26)

Karol Kadlec, Katarzyna Adamska, Adam Voelkel

ABSTRACT

The novel approach for hydroxyapatite (HA) surface characterization was proposed. The main aim of this investigation was to estimate surface properties of HA as a biomaterial in real system i.e. in simulated body fluid (SBF). One of the method, which might be used to reflect the influence of liquid environment on sorption properties of material being surrounded by this liquid, is called inverse liquid chromatography (ILC). The lowercase letters of LFER equation (e, s, a, b, v) served for this characterization. The sorption abilities of examined material were also estimated for two different aqueous mobile phases: deionized water and water solution of 0.1M Na2HPO4. It enabled to observe the change in physiochemical properties of surface, considered in Abraham model, dependence on ions concentration in the mobile phase. Moreover pH of every aquatic solution, normally about 7, was adjusted to 5.5 and 9 to observe the influence of hydrogen and hydroxyl ions concentration on HA sorption properties.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Hydroxyapatite, nanopowder, <200 nm particle size (BET), contains 5 wt. % silica as dopant, synthetic

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

Sigma-Aldrich

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Sigma-Aldrich

Potassium chloride, ≥99.99% trace metals basis

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Sodium chloride solution, 0.85%

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Acetic acid-¹²C₂, 99.9 atom % ¹²C

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Potassium chloride, 99.999% trace metals basis

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Hydroxyapatite, nanoparticles, dispersion, 10 wt. % in H₂O, <200 nm particle size (BET)

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Sodium chloride, random crystals, 99.9% trace metals basis

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Propylamine, ≥99%

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Aniline, ReagentPlus^®, 99%

Sigma-Aldrich

Hydroxyapatite, synthetic, 99.8% trace metals basis (excludes Mg)

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Sodium sulfate, ≥99.99% trace metals basis

Sigma-Aldrich

Trizma^® base, BioUltra, Molecular Biology, ≥99.8% (T)

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Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

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Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H₂O

Sigma-Aldrich

Sodium sulfate, BioUltra, anhydrous, ≥99.0% (T)

Sigma-Aldrich

Trizma^® base, ≥99.0% (T)

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

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Trizma^® base, 99.7% (T), puriss. p.a.

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Potassium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

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Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

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1-Propanol, ≥99%, FG

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Potassium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

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Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

Sigma-Aldrich

1-Propanol, natural, ≥98%, FG

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

Sigma-Aldrich

Aniline, ACS reagent, ≥99.5%

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis