LC/MS Analysis of Ractopamine on SeQuant^® ZIC-HILIC

column

SeQuant^® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Å (1.50661.0001)

mobile phase

[A] acetonitrile; [B] 1.361 g of monopotassium phosphate in 1000 ml water (10mM); (80:20, A:B)

flow rate

1.0 mL/min

pressure

913.5 psi 63 Bar

column temp.

35 °C

detector

306 nm cell: 13 μL

injection

5 μL

sample

50 ppb Ractopamine in mobile phase