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Merck

C0775

Violet cristallisé

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Crystal Violet
1 of 1 reviewers received a sample product or took part in a promotion

Synonyme(s) :

Chlorure d’hexaméthylpararosaniline, Violet 3 de base, Violet de gentiane, Violet de méthyle 10B

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87,50 €

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A propos de cet article

Formule linéaire :
C25H30N3Cl
Numéro CAS:
548-62-9
Poids moléculaire :
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57653980
EC Number:
208-953-6
MDL number:
MFCD00011750
Colour Index Number:
42555
Beilstein/REAXYS Number:
3580948

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Nom du produit

Violet cristallisé, certified by the BSC

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

agency

certified by the BSC

form

powder

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

2.5 — 3.5 (20 °C)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

Quality Level

200

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Cet article
C615846364V5265
Violet cristallisé certified by the BSC

Sigma-Aldrich

C0775

Violet cristallisé

Violet cristallisé ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Violet cristallisé

Violet cristallisé VETRANAL®, analytical standard

Supelco

46364

Violet cristallisé

Crystal Violet Solution 1%, aqueous solution

Sigma-Aldrich

V5265

Crystal Violet Solution

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

microbe id | staining: suitable

grade

certified by the Biological Stain Commission

grade

ACS reagent

grade

analytical standard

grade

-

color

green to very dark green

color

green to very dark green

color

-

color

deep violet, purple

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

H2O: 1 mg/mL

solubility

water: soluble 50 g/L at 27 °C

solubility

water: soluble 50 g/L at 27 °C

solubility

-

form

powder

form

powder

form

-

form

aqueous solution

Application

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability.[1] It has also been used for the staining of cells to study cell migration and invasion.[2]

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA.[3] Crystal violet is also used for the staining of bacteria in gram staining technique.[4] It is also used for the staining of plant chromosomes.[5] Crystal Violet also helps in colorimetric measurement of cell viability.[6]

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000499856
0000499855
0000499855
0000499856
SHBS9966

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
Liao CJ, et al.
Reproduction (Cambridge, England), 147, 179-179 (2013)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
Ki Hyun Kim et al.
PLoS pathogens, 15(2), e1007375-e1007375 (2019-02-02)
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease localized to China, Japan, and Korea that is characterized by severe hemorrhage and a high fatality rate. Currently, no specific vaccine or treatment has been approved for this disease.
Ping Jin et al.
Cell death & disease, 10(8), 615-615 (2019-08-15)
Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide. Development of novel chemotherapeutics is still required to enable successful treatment and improve survival for CRC patients. Here, we found that osimertinib (OSI) exhibits potent anti-CRC effects by
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Questions

1–3 of 3 Questions  

  1. Can crystal violet or Giemsa be used to stain cells in a saliva/PBS suspension for the purpose of counting neutrophils/mL? What would be the best staining method for this purpose?

    1 answer

    1. Acridine orange is theoretically intended to stain viable cells, and if low intensity is observed, particularly when using a light microscope, it is likely true. However, for visualizing viable cells stained with acridine orange, a fluorescent microscope is routinely used. For simple neutrophil counting in saliva, acridine orange may not be the best choice due to cost considerations.

      While Giemsa solutions can be used, staining can be challenging, and often there are no published procedures for using Wright, Wright Giemsa, or Giemsa solution for counting neutrophils under a hemacytometer. The recommended product for this purpose is Crystal Violet or Turks/Tuerks solution, a simple solution of crystal violet dissolved in water. Acetic acid is sometimes added to the Turks solution to lyse red blood cells, but if the saliva is not bloody, adding acetic acid is unnecessary.

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  2. What is the allowed limit of Crystal Violet before it is considered toxic, carcinogenic, or hazardous?

    1 answer

    1. The most serious hazard listed in the Safety Data Sheet (SDS) is Serious Eye Damage, along with short-term and long-term aquatic hazards. There is also a Category 2 hazard as a carcinogen. However, the risk can be reduced by purchasing prepared solutions instead of preparing them from powder to minimize the risk of inhaling the powder. There are no known safe exposure limits for Crystal Violet. The use of appropriate engineering controls, personal protective equipment, and body protection is recommended.

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  3. Can this be dissolved in ethanol or methanol 

    1 answer

    1. While Crystal Violet solutions used for staining often include ethanol or methanol, this product has not been tested for the direct solubility in either alcohol. This compound is readily soluble in water.

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