LC-MS Analysis of Pain Management Opiates using Ascentis® Express Biphenyl
Materials
related product
Product No.
Description
Pricing
(±)-Methadone solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Hydromorphone solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Buprenorphine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Fentanyl solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Hydrocodone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Morphine solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Meperidine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Naloxone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Naltrexone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Oxycodone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Oxymorphone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®cis-Tramadol hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Biphenyl, 5 cm x 2.1mm, 2.7 μm particles (64057-U)
mobile phase
[A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient
10 to 20% B in 1.25 min; to 95% B in 3.75 min
flow rate
0.3 mL/min
pressure
2990 psi (206 bar)
column temp.
30 °C
detector
MS-TOF, ESI+
injection
2 μL
sample
50-500 ng/mL in 99:1, water:methanol
Description
Analysis Note
This application demonstrates the separation of 13 pain managment opiates on the Ascentis Express Biphenyl using an LC-TOF mass spectrometer. This application demonstrates the scalability of this method to 50 x 2.1 mm column geometry using the 2.7 um biphenyl phase. The method provides baseline resolution of all isobaric sets of interest.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany