HTS032RTA
Ready-to-Assay KiSS1 KiSS1-Derived Peptide Receptor Frozen Cells
Human
Se connecter pour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
A propos de cet article
Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.76
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderDescription générale
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
KiSS1 (also known as GPR54 and AXOR12) is a Gq-coupled GPCR that binds to a series of peptides, termed kisspeptins or metastin, derived from the KiSS-1 gene (Kotani et al., 2001; Muir et al., 2001). The KiSS-1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS-1 and GPR54 in inhibition of metastasis in human cancer (Harms et al., 2003). Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus (Messager et al., 2005; Shahab et al., 2005). In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism (Colledge, 2004; Seminara et al., 2004). Millipore′s cloned human KiSS1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant KiSS1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at KiSS1.
KiSS1 (also known as GPR54 and AXOR12) is a Gq-coupled GPCR that binds to a series of peptides, termed kisspeptins or metastin, derived from the KiSS-1 gene (Kotani et al., 2001; Muir et al., 2001). The KiSS-1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS-1 and GPR54 in inhibition of metastasis in human cancer (Harms et al., 2003). Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus (Messager et al., 2005; Shahab et al., 2005). In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism (Colledge, 2004; Seminara et al., 2004). Millipore′s cloned human KiSS1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant KiSS1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at KiSS1.
Application
Human KiSS1 / GPR54 GPCR frozen cells for Calcium Flux FLIPR Assays.
Actions biochimiques/physiologiques
- GPCR Cell Lines
- Host cells: Chem-1
- GPCR Class: A
- Protein Target: KiSS1/ GPR54
- Target Sub-Family: KiSS1-derived peptide
Autres remarques
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Clause de non-responsabilité
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique