H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

• Synonyme(s) : Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
• Formule empirique (notation de Hill) : C₈H₁₈N₂O₄S
• Numéro CAS: 7365-45-9
• Poids moléculaire : 238.30
• UNSPSC Code: 12161704
• NACRES: NA.75
• PubChem Substance ID: 24895731
• EC Number: 230-907-9
• Beilstein/REAXYS Number: 883043
• MDL number: MFCD00006158

Propriétés

• Nom du produit: HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• product line: BioXtra
• assay: ≥99.5% (titration)
• form: powder
• technique(s): cell culture | embryo: suitable
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5; 7.5
• solubility: water: 500 mg/mL, clear, colorless
• absorption: ≤0.05 at 290 in H₂O at 33%
• Quality Level: 400

Description

Application

HEPES has been used:

• As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
• As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Useful pH range: 6.8 - 8.2

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

This product has been qualified for use in cell and mouse embryo culture.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)