The LiChrospher Si 100 (5um) Hibar TS 250-4 HPLC column is suitable for use in normal phase and HILIC modes, as well as SFC. For water-insoluble compounds that are organic solvent soluble, normal phase mode is the mode of choice. The mobile phase for normal phase mode includes n-hexane, n-heptane, dichloromethane, and dichloroethane, with small amounts of diethyl ether, methyl acetate, ethyl acetate, acetone, isopropanol, ethanol, or methanol as modifiers to shorten retention time. An example is 95% hexane/5% ethyl acetate. The combination of solvents used depends on the analytes of interest.
1.50316
LiChrospher® Si 100 (5 µm) HPLC Columns
L x I.D. 250 mm x 4 mm Hibar® RT HPLC Column
Synonym(s):
TM=["LiChrospher"] Si HPLC column
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CHF 788.00
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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
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Product Name
LiChrospher® Si 100 (5 µm) Hibar® RT 250-4, suitable for HPLC
material
stainless steel column
Quality Level
Agency
suitable for USP L3
product line
LiChrospher®
feature
endcapped: no
parameter
400 bar pressure
60 °C max. temp.
technique(s)
HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
25 cm × 4 mm
surface area
350 m2/g
matrix
fully porous particle
matrix active group
Silica bonding phase
particle size
5 μm
pore size
100 Å pore size
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
supercritical fluid (SFC)
storage temp.
no temp limit
Related Categories
1 of 4
This Item | 1.50820 | 1.50830 | 1.50840 |
|---|---|---|---|
| separation technique hydrophilic interaction (HILIC), supercritical fluid (SFC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase |
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 10 μm |
| matrix active group Silica bonding phase | matrix active group Silica bonding phase | matrix active group Silica bonding phase | matrix active group Silica bonding phase |
| pore size 100 Å pore size | pore size 60 Å pore size | pore size 60 Å pore size | pore size 60 Å pore size |
| agency suitable for USP L3 | agency suitable for USP L3 | agency suitable for USP L3 | agency suitable for USP L3 |
| product line LiChrospher® | product line LiChrospher® | product line LiChrospher® | product line LiChrospher® |
General description
HPLC column
Superspher® Si 60 is a versatile HPLC spherical silica sorbent with a particle size of 4µm and a pore diameter of 60A for normal phase chromatography. This sorbent is packed into a Hibar 250-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.
Analysis Note
Pressure: ≤ 80 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 55000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 - 2.0
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 55000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 - 2.0
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany
Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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