Merck
All Photos(2)

10771

Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

NACRES:
NA.75

sterility

sterile-filtered

Quality Level

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.077 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Histopaque-1077 has been used for-
  • the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
  • purification of mononuclear cells through centrifugation
  • isolation of human lymphocytes
  • isolation of breast cancer circulating tumor cells (CTCs) from whole blood

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Comet Assay

The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.

Protocols

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

Comet Assay

The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service