6-[Fluorescein-5(6)-carboxamido]hexanoic acid

Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid
Brand: SIGMA
Price: 327 CHF

suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

Fluorescein-5(6)-carboxamidocaproic acid

Slide 1 of 1

Select a Size

25 MG

CHF 327.00

Check Cart for Availability

Request a Bulk Order

About This Item

Empirical Formula (Hill Notation):

C₂₇H₂₃NO₈

CAS Number:

265981-56-4

Molecular Weight:

489.47

MDL number:

MFCD29074000

UNSPSC Code:

12352106

PubChem Substance ID:

57650886

NACRES:

NA.32

Key Documents

View All Documentation

Product Name

6-[Fluorescein-5(6)-carboxamido]hexanoic acid, suitable for fluorescence, ≥90% (HPCE)

SMILES string

OC(=O)CCCCCNC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35.OC(=O)CCCCCNC(=O)c6ccc7C(=O)OC8(c9ccc(O)cc9Oc%10cc(O)ccc8%10)c7c6

InChI

1S/2C27H23NO8/c29-16-6-9-20-22(13-16)35-23-14-17(30)7-10-21(23)27(20)19-8-5-15(12-18(19)26(34)36-27)25(33)28-11-3-1-2-4-24(31)32;29-16-6-9-19-22(13-16)35-23-14-17(30)7-10-20(23)27(19)21-12-15(5-8-18(21)26(34)36-27)25(33)28-11-3-1-2-4-24(31)32/h2*5-10,12-14,29-30H,1-4,11H2,(H,28,33)(H,31,32)

InChI key

WEJBWDKWIFHWDE-UHFFFAOYSA-N

assay

≥90% (HPCE)

form

solid

solubility

DMF: soluble
DMSO: soluble

fluorescence

λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

200

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	46939	21878	46940
Sigma-Aldrich 46935 6-[Fluorescein-5(6)-carboxamido]hexanoic acid	Sigma-Aldrich 46939 Fluorescein diacetate 5-maleimide	Sigma-Aldrich 21878 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester	Sigma-Aldrich 46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
suitability suitable for fluorescence	suitability -	suitability suitable for fluorescence	suitability suitable for fluorescence
fluorescence λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0	fluorescence λ_ex 492 nm; λ_em 520 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol and after cleavage by esterase)	fluorescence λ_ex 492 nm; λ_em 517 nm in DMF	fluorescence λ_ex 490 nm; λ_em 514 nm (pH 8.2), λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0
form solid	form solid	form powder	form solid
solubility DMF: soluble, DMSO: soluble	solubility DMF: soluble, DMSO: soluble, dioxane: soluble	solubility DMF: soluble, DMSO: soluble	solubility DMF: soluble, methanol: soluble
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 100

Application

6-[Fluorescein-5(6)-carboxamido]hexanoic acid is used as a fluorescein-based fluorescent label for proteins that can be analyzed in applications such as quenching experiments.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Production and properties of skeletal myosin subfragment 1 selectively labeled with fluorescein at lysine-553 proximal to the strong actin-binding site.

Bertrand R, Derancourt J, Kassab R.

Biochemistry, 3, 9500-9507 (1995)

The antimicrobial peptide Temporin L impairs E. coli cell division by interacting with FtsZ and the divisome complex.

Angela Di Somma et al.

Biochimica et biophysica acta. General subjects, 1864(7), 129606-129606 (2020-04-02)

The comprehension of the mechanism of action of antimicrobial peptides is fundamental for the design of new antibiotics. Studies performed looking at the interaction of peptides with bacterial cells offer a faithful picture of what really happens in nature. In

2,4-Dinitrophenol reduces the reactivity of Lys553 in the lower 50-kDa region of myosin subfragment 1.

Theresa R Bomfim et al.

Archives of biochemistry and biophysics, 505(1), 105-111 (2010-10-05)

2,4-Dinitrophenol (DNP) increases the affinity of myosin for actin and accelerates its Mg(2+)ATPase activity, suggesting that it acts on a region of the myosin head that transmits conformational changes to actin- and ATP-binding sites. The binding site/s for DNP are

Liver cell uptake and degradation of soluble immunoglobulin G immune complexes in vivo and in vitro in rats.

A G Johansson et al.

Hepatology (Baltimore, Md.), 24(1), 169-175 (1996-07-01)

Immune complexes were formed between dinitrophenylated human serum albumin (DNP-HSA) and polyclonal rabbit immunoglobulin G (IgG) anti-DNP antibodies at antibody excess. The antigen was labelled with isotope (125I-tyramine-cellobiose) or fluorochrome, (6-[fluorescein-5-(and-6)-carboxamido] hexanoic-acid, succinimidyl ester). The radiolabelled antigen, native or antibody

Dynamics at Lys-553 of the acto-myosin interface in the weakly and strongly bound states.

J J MacLean et al.

Biophysical journal, 78(3), 1441-1448 (2000-02-29)

Lys-553 of skeletal muscle myosin subfragment 1 (S1) was specifically labeled with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) and fluorescence quenching experiments were carried out to determine the accessibility of this probe at Lys-553 in both the

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service