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B4500

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Synonym(s):

BAEE

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About This Item

Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
2645-08-1
Molecular Weight:
342.82
NACRES:
NA.32
PubChem Substance ID:
24891777
UNSPSC Code:
12352204
EC Number:
220-157-0
MDL number:
MFCD00012579
Beilstein/REAXYS Number:
3781694

Key Documents

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Product Name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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This Item
B3279B3133B4750
Nα-Benzoyl-L-arginine ethyl ester hydrochloride trypsin substrate

Sigma-Aldrich

B4500

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride ≥98% (HPLC)

Sigma-Aldrich

B3279

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride ≥99% (TLC), suitable for substrate for trypsin

Sigma-Aldrich

B3133

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride trypsin substrate

Sigma-Aldrich

B4750

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride

assay

≥97% (HPLC)

assay

>98% (TLC)

assay

≥99% (TLC)

assay

≥98% (TLC)

solubility

water: 50 mg/mL, clear, colorless

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

acetone: water (1:1): 50 mg/mL

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles[1]
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts[2]
  • in trypsin activity assay of the tail tendon fascicles from rat[3]

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin.[4] It is a nonprotein substrate used in spectroscopic assays.[5]
The prototype substrate for trypsin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000499113
0000499113
BCCN7609
BCCN5707
0000396931

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Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
The mechanical, structural, and compositional changes of tendon exposed to elastase
Grant TM, et al.
Annals of Biomedical Engineering, 43(10), 2477-2486 (2015)
Weijun Wang et al.
Scientific reports, 9(1), 13630-13630 (2019-09-22)
Cellulases play important roles in the dietary fibre digestion in pigs, and have multiple industrial applications. The porcine intestinal microbiota display a unique feature in rapid cellulose digestion. Herein, we have expressed a cellulase gene, p4818Cel5_2A, which singly encoded a
Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
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Protocols

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

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