C0739

Anti-phospho-Cortactin (pTyr⁴²¹) antibody produced in rabbit

Name: Anti-phospho-Cortactin (pTyr<SUP>421</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 722 CHF

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:

MFCD04975269

UNSPSC Code:

12352203

NACRES:

NA.41

Key Documents

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 80-85 kDa

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
western blot: 1:1,000

UniProt accession no.

Q60598

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr421)

Gene Information

mouse ... Cttn(13043)

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This Item	SAB4504373	C6987	SAB4504372
Sigma-Aldrich C0739 Anti-phospho-Cortactin (pTyr⁴²¹) antibody produced in rabbit	Sigma-Aldrich SAB4504373 Anti-phospho-Cortactin (pTyr⁴⁶⁶) antibody produced in rabbit	$Anti-Cortactin (GK-18) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C6987 Anti-Cortactin (GK-18) antibody produced in rabbit	Sigma-Aldrich SAB4504372 Anti-phospho-Cortactin (pTyr⁴²¹) antibody produced in rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
technique(s) immunocytochemistry: suitable, western blot: 1:1,000	technique(s) ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) indirect immunofluorescence: 1:500 using mouse fibroblasts NIH3T3 cell line, microarray: suitable, western blot: 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of human epidermoid carcinoma A431 cell line	technique(s) ELISA: 1:20000, western blot: 1:500-1:1000
species reactivity mouse	species reactivity human, rat, mouse	species reactivity human, mouse, rat, chicken	species reactivity human, mouse, rat

General description

Cortactin is encoded by the CTTN gene on chromosome 11 in humans. It is a cytoskeleton protein that facilitates assembly of cortical actin. It is expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src.

Immunogen

synthetic phosphopeptide derived from the region of mouse cortactin that is phosphorylated on tyrosine 421.

Application

Anti-phospho-Cortactin (pTyr⁴²¹) antibody produced in rabbit is suitable for immunocytochemistry and western blotting at a working dilution of 1:1,000

Biochem/physiol Actions

Cortactin is activated by phosphorylation of tyrosine kinases or serine/threonine kinases. It plays an important role in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis. Cortactin regulates the interactions between components of adherens-type junctions and helps in organizing the cytoskeleton, cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. It acts as a regulator in invasion and migration of tumor cells. The overexpression of cortactin causes osteosarcoma carcinogenesis and along with FAK increases the risk of oral premalignant lesions.

Physical form

Solution in Dulbecco′s PBS, pH 7.3, 1mg/mL BSA, 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Mena

Maxwell D Weidmann et al.

Scientific reports, 6, 36142-36142 (2016-11-09)

Invadopodia, actin-based protrusions of invasive carcinoma cells that focally activate extracellular matrix-degrading proteases, are essential for the migration and intravasation of tumor cells during dissemination from the primary tumor. We have previously shown that cortactin phosphorylation at tyrosine residues, in

Invadopodia in context.

Aviv Bergman et al.

Cell adhesion & migration, 8(3), 273-279 (2014-04-10)

Invadopodia are dynamic protrusions in motile tumor cells whose function is to degrade extracellular matrix so that cells can enter into new environments. Invadopodia are specifically identified by microscopy as proteolytic invasive protrusions containing TKS5 and cortactin. The increasing complexity

Cortactin is a sensitive biomarker relative to the poor prognosis of human hepatocellular carcinoma.

Gang Zhao et al.

World journal of surgical oncology, 11, 74-74 (2013-03-23)

Cortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC). The aim of this study was to elucidate the forecasting role of cortactin in resectable HCCs. We compared the invasiveness and motility among liver epithelial cell

Targeting invadopodia-mediated breast cancer metastasis by using ABL kinase inhibitors.

Tomer Meirson et al.

Oncotarget, 9(31), 22158-22183 (2018-05-19)

Metastatic dissemination of cancer cells from the primary tumor and their spread to distant sites in the body is the leading cause of mortality in breast cancer patients. While researchers have identified treatments that shrink or slow metastatic tumors, no

Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex.

S A Weed et al.

The Journal of cell biology, 151(1), 29-40 (2000-10-06)

Cortactin is an actin-binding protein that is enriched within the lamellipodia of motile cells and in neuronal growth cones. Here, we report that cortactin is localized with the actin-related protein (Arp) 2/3 complex at sites of actin polymerization within the

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Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit