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G6392
L-Glutamine
γ-irradiated, BioXtra, suitable for cell culture
Synonym(s):
(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
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1 VIAL
CHF 24.70
10 VIALS
CHF 280.00
About This Item
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
EC Number:
200-292-1
NACRES:
NA.75
Beilstein/REAXYS Number:
1723797
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Product Name
L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
sterility
γ-irradiated
product line
BioXtra
form
powder
packaging
vial of 0.292 g
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white to faint yellow
mp
185 °C (dec.) (lit.)
solubility
H2O: 10 mL/vial, clear to slightly hazy
Quality Level
Gene Information
rat ... Ggt1(116568)
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This Item | G8540 | G3126 | 49419 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| product line BioXtra | product line - | product line ReagentPlus® | product line BioUltra |
| solubility H2O: 10 mL/vial, clear to slightly hazy | solubility H2O: 25 mg/mL | solubility 1 M HCl: 50 mg/mL, clear to slightly hazy, colorless | solubility H2O: 0.1 M at 20 °C, clear, colorless |
| mp 185 °C (dec.) (lit.) | mp 185 °C (dec.) (lit.) | mp 185 °C (dec.) (lit.) | mp 185 °C (dec.) (lit.) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 100 |
Application
L-Glutamine has been used as a media supplement:
- for cochlear organotypic culturing[1]
- in Iscove′s modified Dulbecco′s medium for HeLa cells culturing[2]
- in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing[3]
Qualified for use in cell culture applications, especially serum-free cell culture.
Biochem/physiol Actions
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.[4]
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.
Other Notes
The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.
Packaging
50 mL amber serum vial with rubber stopper.
Preparation Note
Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.
related product
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Nonresonant confocal Raman imaging of DNA and protein distribution in apoptotic cells
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Christoffersen C, et al.
The Journal of Biological Chemistry, 283(27), 18765-18772 (2008)
Cisplatin ototoxicity in rat cochlear organotypic cultures
Ding D, et al.
Hearing Research, 282(1-2), 196-203 (2011)
Rebekah M Samsonraj et al.
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Articles
Importance and uses of glutamine in hybridoma and mammalian cell culture
Bedeutung und Verwendung von Glutamin in der Hybridom- und Säugerzellkultur
Protocols
MTT-Assayprotokoll für die Messung von Zellviabilität, Zellproliferation und Zytotoxizität. Anwendungshinweise für die MTT Reagenzvorbereitung und Beispiele für Anwendungen.
Durchführung nicht-radioaktiver Quantifizierung von Zellproliferation, Viabilität und Zytotoxizität adhärenter oder Suspensionszellen, die in 96-Well-Mikrotiterplatten kultiviert werden, mittels colorimetrischer Assays.
WST-1-Assayprotokoll für die Messung von Zellviabilität, Zellproliferation, Aktivierung und Zytotoxizität. Anwendungshinweise für die WST-1 Reagenzvorbereitung und Beispiele für Anwendungen. Häufig gestellte Fragen und Anleitung für die Fehlersuche für den WST-1 Assay
Protocole du test au MTT pour la mesure de la viabilité, de la prolifération et de la toxicité cellulaires. Instructions de préparation du réactif au MTT et exemples d'applications.
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What is the Department of Transportation shipping information for this product?
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
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Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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