G7654
Gel Loading Solution
for NA electrophoresis, solution
Synonym(s):
DNA Gel Loading Solution, Gel Loading Buffer
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5 ML
CHF 30.30
CHF 30.30
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161703
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Product Name
Gel Loading Solution, for NA electrophoresis, solution
grade
Molecular Biology
form
solution
concentration
6 ×
storage temp.
2-8°C
Quality Level
1 of 4
This Item | G2526 | R1386 | R4268 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| concentration 6 × | concentration - | concentration - | concentration 1.25 × |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form solution | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. −20°C |
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
General description
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Other Notes
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
Preparation Note
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
related product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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