The volume may be determined using the lot specific Enzymatic Activity reported in the Certificate of Analysis. For example, if a specific lot reports an Enzymatic Activity of 7.17 U/mL, the 1 unit pack size will have a volume of roughly 139 uL and the 2 unit pack size would have a volume of 278 uL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/n7885#product-documentation
N7885
Neuraminidase from Vibrio cholerae
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
Synonym(s):
Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
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1 UNIT
CHF 182.00
2 UNITS
CHF 488.00
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
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Product Name
Neuraminidase from Vibrio cholerae, Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
type
Type III
form
buffered aqueous solution
specific activity
1-5 units/mg protein (Lowry, using NAN-lactose)
mol wt
83 kDa
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
storage temp.
2-8°C
Quality Level
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Related Categories
1 of 4
This Item | N2876 | N2133 | N5631 |
|---|---|---|---|
| specific activity 1-5 units/mg protein (Lowry, using NAN-lactose) | specific activity ≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA) | specific activity ≥50 units/mg protein (using 4MU-NANA) | specific activity 10-20 units/mg protein (using 4MU-NANA), 3.5-8.0 units/mg protein (mucin) |
| form buffered aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| mol wt 83 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Analysis Note
Preservative free.
Application
Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. [1] It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. [2]
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.
Biochem/physiol Actions
Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. [1]
General description
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Other Notes
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
Physical form
Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.
Preparation Note
Chromatographically purified.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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J E Galen et al.
Infection and immunity, 60(2), 406-415 (1992-02-01)
Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing the binding and penetration of CT to enterocytes. To test this hypothesis, the NANase gene (nanH) from
P C Reading et al.
Journal of virology, 74(11), 5190-5197 (2000-05-09)
Influenza viruses A/PR/8/34 (PR8; H1N1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJx109; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
M Potier et al.
Analytical biochemistry, 94(2), 287-296 (1979-04-15)
Jai Woong Seo et al.
Nature communications, 11(1), 2102-2102 (2020-05-02)
Adeno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids. Recently, tissue-specific AAV capsids (e.g. PHP.eB) have been shown to enhance brain delivery in rodents via the LY6A receptor on brain endothelial cells. Here, we create
Paola Rota et al.
Organic & biomolecular chemistry, 10(14), 2885-2894 (2012-03-08)
A simple protocol for the synthesis of N-perfluoroacylated and N-acylated glycals of neuraminic acid, with a secondary cyclic amine (morpholine or piperidine) at the 4α position, has been set-up, starting from peracetylated N-acetylneuraminic acid methyl ester that undergoes, sequentially to
Articles
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Protocols
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
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Instructions
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Dear Customer service I have a question regarding the product (N7885 Neuraminidase from Vibrio cholerae). In how much volume does the 1 Unit or 2 Unit package arrive? Could you provide a volume (or mg if it's powder)? Thanks, Ben
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