RDRT
ReadyScript® cDNA Synthesis Mix
Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym(s):
cDNA Synthesis Mix
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About This Item
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usage
sufficient for 100 reactions
sufficient for 25 reactions
sufficient for 500 reactions
feature
dNTPs included
hotstart: no
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | P4600 | 11483188001 | FPCR-RO |
|---|---|---|---|
| technique(s) RT-PCR: suitable | technique(s) PCR: suitable | technique(s) RT-PCR: suitable | technique(s) PCR: suitable |
| usage sufficient for 100 reactions | usage sufficient for 100 reactions | usage sufficient for 30 reactions (including 5 control reactions) | usage sufficient for ≤2,000 reactions (04710452001), sufficient for ≤100 reactions (04710436001), sufficient for ≤400 reactions (04710444001) |
| feature dNTPs included | feature dNTPs included, hotstart: no | feature dNTPs included, hotstart: no | feature - |
| color colorless | color colorless | color - | color - |
| input purified RNA | input purified DNA | input purified RNA | input - |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
General description
Application
Features and Benefits
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
- Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
- Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
- Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
- Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month
- Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
- High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
- RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
- Reverse transcribes both rare and abundant genes with a wide input range of template
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
Other Notes
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protocols
Il nostro protocollo SYBR Green qPCR è un metodo progettato per la quantificazione accurata dell'espressione genica e delle reazioni RT-PCR.
Notre protocole de qPCR avec SYBR Green est conçu pour quantifier précisément l'expression génique ou des réactions de RT-PCR.
Reverse Transkription ist die Analyse der Genexpression zur Messung der mRNA-Konzentration eines Gens. Eine solche Analyse birgt mehrere Herausforderungen, wie z. B. die unterschiedliche Halbwertszeit der verschiedenen Transkripte, die zeitlichen Muster der Transkription und die fehlende Korrelation zum Protein.
Unser SYBR Green qPCR-Protokoll ist eine Methode zum Nachweis einer genauen Quantifizierung der Genexpression und der RT-PCR-Reaktionen.
Related Content
La réaction en chaîne par polymérase (PCR pour "Polymerase Chain Reaction") est une technique d'amplification des molécules d'acide nucléique qui est couramment employée dans de nombreuses applications, incluant la RT-PCR, la PCR à démarrage à chaud, la PCR en point final, et bien d'autres.
La RT-qPCR, ou PCR quantitative après transcription inverse, combine les effets de la transcription inverse et de la PCR quantitative, ou PCR en temps réel, pour amplifier et détecter des cibles spécifiques. La RT-qPCR trouve de multiples applications, notamment dans la quantification du niveau d'expression génique, la validation de l'interférence par ARN ou la détection d'agents pathogènes comme les virus.
La PCR (reazione a catena della polimerasi) è una tecnica che consente di amplificare le molecole di acido nucleico comunemente usata in molte applicazioni fra cui RT-PCR, hot start PCR, end point PCR ed altre.
In der RT-qPCR oder quantitativen PCR mit reverser Transkription werden die Effekte der reversen Transkription und der quantitativen PCR oder Echtzeit-PCR für die Amplifikation und den Nachweis spezifischer Ziele kombiniert. Die RT-qPCR wird in vielen Anwendungen eingesetzt, darunter die Quantifizierung der Genexpressionsniveaus, die Validierung der RNA-Interferenz (RNAi) und der Nachweis von Krankheitserregern wie Viren.
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