RIP

Sigma-Aldrich

Imprint® RNA Immunoprecipitation Kit

High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

NACRES:
NA.54

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General description

RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.

Learn more about this product and view workflow and application data.

Application

Imprint® RNA Immunoprecipitation Kit has been used for:
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Xtra Protein A magnetic beads with higher binding capacity
  • Step-by-step protocols for cell lysis and immunoprecipitation
  • RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
  • Negative control and antibody included

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  • R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL

pictograms

FlameExclamation mark

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Warning

hazcat

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

storage_class_code

3 - Flammable liquids

Flash Point F

100.4 °F

Flash Point C

38 °C

Certificate of Analysis

Certificate of Origin

Yu-Yuan Qian et al.
Oncotarget, 8(64), 107859-107869 (2018-01-04)
Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, was previously reported to be a tumour suppressor in some cancer types. However, the precise effects mediated by PTENP1 transcripts within intricate regulatory networks involving molecular interactions with PTEN and...
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The...
Nicola Amodio et al.
Leukemia (2018-03-01)
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance and the druggability of the oncogenic lncRNA MALAT1 in MM. Targeting MALAT1 by novel...
Alex Michael Ward et al.
Scientific reports, 6, 34379-34379 (2016-09-30)
Dengue virus (DENV) is a mosquito-borne Flavivirus classified into four serotypes (DENV-1-4) that causes Dengue fever (DF), Dengue hemorrhagic Fever (DHF) or Dengue shock syndrome (DSS). An estimated 390 million people are at risk for infection with DENV and there...
Long non-coding RNA HOTAIR is targeted and regulated by miR-141 in human cancer cells.
Chiyomaru T, et al.
The Journal of Biological Chemistry, 289(19), 12550-12565 (2014)
Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
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Protocols
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Read More

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