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Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover.

Bioresource technology (2012-05-23)
Qin Chen, Megan N Marshall, Scott M Geib, Ming Tien, Tom L Richard
ABSTRACT

The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetramethylammonium hydroxide pentahydrate, ≥97%
Sigma-Aldrich
Tetramethylammonium bromide, 98%
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Tetramethylammonium bromide, ACS reagent, ≥98.0%
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Tetramethylammonium iodide, 99%
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Tetramethylammonium nitrate, 95%
Supelco
Tetramethylammonium bromide, suitable for ion pair chromatography, LiChropur, ≥99.0% (AT)
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Tetramethylammonium hydroxide pentahydrate, ≥95.0% (T)
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Tetramethylammonium chloride solution, for molecular biology
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Tetramethylammonium fluoride, 97%
Sigma-Aldrich
Tetramethylammonium hydroxide solution, ACS reagent
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Tetramethylammonium hydroxide solution, 25 wt. % in H2O
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Tetramethylammonium hydroxide solution, 10 wt. % in H2O
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Tetramethylammonium chloride, reagent grade, ≥98%
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Tetramethylammonium chloride, BioUltra, for molecular biology, ≥99.0% (AT)
Supelco
Tetramethylammonium sulfate, suitable for ion pair chromatography, LiChropur, ≥99.0% (T)
Sigma-Aldrich
Tetramethylammonium hydroxide solution, 25 wt. % in methanol
Supelco
Tetramethylammonium chloride, suitable for ion pair chromatography, LiChropur, ≥99.0% (AT)