Merck
  • Home
  • Search Results
  • Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.

Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014-04-30)
Mohamad F Aslam, David M Frazer, Nuno Faria, Sylvaine F A Bruggraber, Sarah J Wilkins, Cornel Mirciov, Jonathan J Powell, Greg J Anderson, Dora I A Pereira
ABSTRACT

The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO4), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Supelco
Iron(III) citrate tribasic monohydrate, 18-20% Fe basis (T)
Sigma-Aldrich
Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Iron(III) citrate, technical grade
Sigma-Aldrich
Ferric citrate, BioReagent, suitable for cell culture
SAFC
Ferric citrate
Sigma-Aldrich
Iron(II) sulfate heptahydrate, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.5-104.5% (manganometric)
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ReagentPlus®, ≥99%
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Giemsa stain, technical grade, used as a blood stain
Sigma-Aldrich
Giemsa stain, certified by the Biological Stain Commission
Sigma-Aldrich
Adipic acid, BioXtra, ≥99.5% (HPLC)
Supelco
Adipic acid, certified reference material, TraceCERT®
Adipic acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Adipic acid, 99%
Sigma-Aldrich
Adipic acid, ≥99.5%