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Protective effects of polyphenols-enriched extract from Huangshan Maofeng green tea against CCl4-induced liver injury in mice.

Chemico-biological interactions (2014-06-29)
Yanmang Cui, Xingbin Yang, Xinshan Lu, Jinwen Chen, Yan Zhao
ABSTRACT

The study was to characterize the polyphenolic composition, antioxidant properties, and hepatoprotective effects of a polyphenols-enriched extract (HMTP) from Huangshan Maofeng green tea. HPLC analysis showed that three predominantly polyphenolic compounds present in HMTP were epigallocatechin (271.2 μg/mg extract), rutin (239.3 μg/mg) and epicatechin (89.3 μg/mg). HMTP was shown to exhibit strong scavenging activities against DPPH, O2(-), and OH, and ferric-reducing antioxidant power in vitro. Administration of HMTP at 200, 400 and 800 mg/kg bw in mice prior to CCl4 injury significantly decreased the CCl4-induced elevation of serum ALT, AST and ALP activities, and prevented an increase in hepatic MDA levels (p<0.05). Mice with HMTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH-Px and SOD activities in the liver, relative to CCl4-intoxicated mice. Liver pathological observation also confirmed the protection on CCl4-caused histological alteration, suggesting that HMTP has potential to be explored as valuable hepatoprotective function food.

MATERIALS
Product Number
Brand
Product Description

USP
Quercetin, United States Pharmacopeia (USP) Reference Standard
Supelco
(−)-Epicatechin, analytical standard
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Supelco
Quercetin, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Glutathione, Pharmaceutical Secondary Standard; Certified Reference Material
Glutathione, European Pharmacopoeia (EP) Reference Standard
Chlorogenic acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Carbon tetrachloride, reagent grade, 99.9%
Supelco
Carbon tetrachloride, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Edelfosine, ≥95% (HPLC)
Epicatechin, primary reference standard
Epicatechin gallate, primary reference standard
USP
Butylated hydroxytoluene, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Trichloroacetic acid, ≥99.0% (titration)
Sigma-Aldrich
(−)-Epicatechin, ≥98% (HPLC), from green tea
Sigma-Aldrich
Trichloroacetic acid, BioXtra, ≥99.0%
Sigma-Aldrich
Nitrotetrazolium Blue chloride, powder, electrophoresis grade
Supelco
4,4′-Diaminodiphenylmethane, analytical standard
Sigma-Aldrich
Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
Sigma-Aldrich
Nitrotetrazolium Blue chloride, ≥90.0% (HPLC)
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
(−)-Epicatechin gallate, ≥98% (HPLC), from green tea
Sigma-Aldrich
(−)-Epicatechin, ≥90% (HPLC)
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
Sigma-Aldrich
Trichloroacetic acid, puriss. p.a., ACS reagent, ≥99.5%
Sigma-Aldrich
4,4′-Diaminodiphenylmethane, ≥97.0% (GC)
Supelco
Carbon tetrachloride, analytical standard
Sigma-Aldrich
Trichloroacetic acid, BioUltra, ≥99.5% (T)
Hyperoside, primary reference standard