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Imprint® Ultra Chromatin Optimization Kit

Kit designed to optimize sonication parameters for ChIP experiments

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NACRES:
NA.54
UNSPSC Code:
41116158
eCl@ss:
32160701
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General description

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

Other Notes

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
CHP12015917409
Imprint&#174; Ultra Chromatin Optimization Kit Kit designed to optimize sonication parameters for ChIP experiments

Sigma-Aldrich

CHROP

Imprint® Ultra Chromatin Optimization Kit

Imprint&#174; Chromatin Immunoprecipitation Kit Complete ChIP reaction in 6 hours in flexible strip well format

Sigma-Aldrich

CHP1

Imprint® Chromatin Immunoprecipitation Kit

5M NaCl - for use in Chromatin Immunoprecipitation (ChIP) 5M NaCl -for use in Chromatin Immunoprecipitation (ChIP).

Sigma-Aldrich

20159

5M NaCl - for use in Chromatin Immunoprecipitation (ChIP)

EZ-Magna ChIP&#174; G - Chromatin Immunoprecipitation Kit Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic G beads. Control primers included.

Sigma-Aldrich

17409

EZ-Magna ChIP® G - Chromatin Immunoprecipitation Kit

description

Kit designed to optimize sonication parameters for ChIP experiments

description

Complete ChIP reaction in 6 hours in flexible strip well format

description

5M NaCl -for use in Chromatin Immunoprecipitation (ChIP).

description

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic G beads. Control primers included.

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

Signal Word

Danger

Target Organs

Central nervous system

Storage Class

5.1A - Strongly oxidizing hazardous materials

Flash Point (°F)

100.4 °F

Flash Point (°C)

38 °C

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3

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