MITOISO1
Mitochondria Isolation Kit
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Synonyma:
Mitochondria Purification Kit
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O této položce
UNSPSC Code:
12352200
EC Number:
232-650-8
NACRES:
NA.32
eCl@ss:
32160702
Technický servis
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usage
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL)
shelf life
2 yr
technique(s)
fractionation: suitable
input
mammalian sample(s)
shipped in
dry ice
storage temp.
−20°C
Application
Mitochondria Isolation Kit has been used:
- for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
- for the isolation of mitochondria from glomeruli and heart
- for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential
The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Features and Benefits
- Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
- Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
- Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
- Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane
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Výstražné slovo
Danger
Kódy nebezpečí
Bezpečnostní pokyny
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 - vPvM
Cílové orgány
Respiratory system
Skladovací třída
10 - Combustible liquids
WGK
WGK 3
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