219475

CENTA β-Lactamase Substrate

≥98% (TLC), powder, β-lactamase substrate, Calbiochem^®

• Synonym(s): CENTA β-Lactamase Substrate
• CAS Number: 80072-86-2
• UNSPSC Code: 12352200
• NACRES: NA.54
• Assay: ≥98% (TLC)
• Form: powder
• Storage condition: OK to freeze

Properties

• Product Name: CENTA β-Lactamase Substrate, CENTA β-Lactamase Substrate is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.
• Quality Segment: 100
• description: RTECS - OD2265000
• assay: ≥98% (TLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: yellow
• solubility: 25 mM phosphate buffer, pH 7.0: 2.5 mg/mL, methanol: 50 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Chromogenic β-lactamase substrate for both gram-negative and gram-positive bacteria that is comparable to other chromogens (PADAC Substrate and nitrocefin). Hydrolysis of the beta-lactam ring causes a color change from light yellow (λ_max: 340 nm) to chrome yellow (λ_max: 405 nm). Relatively unaffected by commonly used microbiological media and human serum. Stable in H₂O and in the presence of cysteine, mercaptoethanol and dimercaptopropanol.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.N., et al. 1982. J. Clin. Microbiol.15, 954.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable